Journal article
X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age
A Grubman, P Guennel, KA Vessey, MWM Jones, SA James, MD De Jonge, AR White, EL Fletcher
Metallomics | ROYAL SOC CHEMISTRY | Published : 2016
DOI: 10.1039/c6mt00055j
Abstract
The biologically important metals such as zinc, copper and iron play key roles in retinal function, yet no study has mapped the spatio-temporal distribution of retinal biometals in healthy or diseased retina. We investigated a natural mouse model of retinal degeneration, the Cln6nclf mouse. As dysfunctional metabolism of biometals is observed in the brains of these animals and deregulated metal homeostasis has been linked to retinal degeneration, we focused on mapping the elemental distribution in the healthy and Cln6nclf mouse retina with age. Retinal and RPE elemental homeostasis was mapped in Cln6nclf and C57BL6/J mice from 1 to 8 months of age using X-ray Fluorescence Microscopy at the A..
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Awarded by Australian Research Council
Funding Acknowledgements
Parts of this work were performed at the XFM beamline at the Australian Synchrotron, Victoria, Australia. We would like to thank Tony Grubman for his assistance with data analysis. This work was supported by the National Health and Medical Research Council of Australia (NHMRC, ELF and KV APP1061419) and Australian Research Council (ARC). A. G. is funded by a Melbourne Neuroscience Institute Fellowship. A. G. and S. A. J. are funded by the NHMRC-ARC Dementia Development Research Fellowship. The funding sources had no influence in study design; collection, analysis, and interpretation of data; writing the report; or the decision to submit the report for publication.